MFLP-41 Environmental Sampling for the Detection of Microorganisms
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ID： |
DB646A5325984B48975160F0056632D8 |
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文件大小(MB)： |
0.13 |
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页数： |
11 |
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文件格式： |
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日期： |
2012-3-2 |
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文本摘录（文本识别可能有误，但文件阅览显示及打印正常，pdf文件可进行文字搜索定位）：
Published on the Food Directorate’s (Health Canada's) website at:,http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/index-eng.php,Laboratory Procedure MFLP-41,July 2010,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,ENVIRONMENTAL SAMPLING FOR THE,DETECTION OF MICROORGANISMS,micro_methods_committee@hc-sc.gc.ca,1. APPLICATION,This procedure is applicable to the sampling of the environment for microorganisms in food,establishments in support of compliance activities relative to Section 7 of the Food and Drugs Act,and Health Canada policies such as the Policy on Listeria monocytogenes in ready-to-eat (RTE),Food and the Canadian Food Inspection Agency Directives (CFIA) and guidelines. This,environmental sampling procedure applies to both food contact surfaces (FCS) and non-food,contact surfaces (NFCS) in a food manufacturing plant. It describes the procedures to be used,for the collection and handling of environmental samples for microbiological assessment. This,revised procedure replaces MFLP-41A and MFLP-41B dated September 1992 and July 2006,respectively.,2. PRINCIPLE,With a well designed sampling plan, analysis of environmental samples will aid in demonstrating,whether there is adequate control over good manufacturing practices and if the sanitization,process is adequate. Environmental sampling is an important tool to verify if there is a need for,additional pathogen control measures, provides information over time for trend analysis, and,provides some measure of confidence that products being manufactured are made under sanitary,conditions. If a quantitative method is used, it is essential that microbiological analysis be initiated,as soon as possible after sampling in order to avoid any loss due to die-off of viable,microorganisms.,3. BACKGROUND,Environmental sampling for the detection of microorganisms was first used by the Health Canada,during the 1970s to investigate Salmonella contamination in milk powder plants. It can be used to,determine the presence of pathogens, as well as indicator microorganisms (e.g., coliforms) on,equipment, food contact and non-food contact surfaces. It is now recognized that bacteria can,grow on food contact surfaces and may form biofilms which may be very difficult to remove during,routine cleaning operations.,Microbiological results of environmental sampling can be used by inspectors to document,deviations from good manufacturing practices, for enforcement purposes and as a tool to,MFLP-41,July 2010,- 2 -,demonstrate where corrective measures are needed. It is important that an appropriate,standardized technique be used by government and industry across the country to maintain,uniformity.,4. BEFORE SAMPLING,4.1 Before commencing the environmental sampling, the person responsible should take the,following steps:,a) Draw a process flow chart and plant traffic pattern;,b) Determine target organism(s),c) Identify the critical control points;,d) Determine sampling objectives (e.g., FCS or NFCS),e) Arrange for sterile equipment and material as required.,f) Identify the sampling material and record according to sampling sites (e.g., label,each sample that is taken with the date, site sampled, FCS / NFCS).,g) Identify other hazards associated with the sampling area.,4.2 Aerobic colony counts and other indicator microorganisms (e.g., faecal coliforms) can be,used to assess overall sanitation.,If information is needed regarding cleaning and sanitation, it is recommended that,samples be taken immediately before production, just after cleaning and sanitation have,been completed.,If information is needed regarding the microbiological environmental conditions during,production, it is recommended that samples be taken during production. Consult with the,appropriate regulatory program guidelines from the CFIA, provinces, etc. for specific,instructions that may apply.,5. MATERIALS,Requirements will vary depending on the method of sampling, the target organism(s) and the,analytical method(s) which will be employed. For specific microbial media requirements, refer to,the Compendium of Analytical Methods, Annex G (11.4).,5.1 Sterile material may be purchased or prepared by laboratory personnel,Commercially available environment sampling kits for food establishments or in-house,kits may also be used. Sterile material in the kit must be assembled using aseptic,technique. The kit should include swabs, sterile gloves, neutralizing buffer, marker, ice,packs, and sealable plastic bags (e.g., Whirl-pak.).,MFLP-41,July 2010,- 3 -,Non-sterile equipment used for sampling should be sanitized before sample collection,(e.g., cooler, sampling boxes, ice packs).,5.2 Surface……
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